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Exercise increases plasma tryptophan availability, facilitating its transport into the brain, where it is converted into serotonin and increasing the activity of TPH, the rate-limiting enzyme in serotonin synthesis [245]

OPLS-DA is a supervised classification technique and maximizes the covariance between the measured data of the peak intensities in NMR spectra (the X-variable) and the response of the class assignment (Y-variable) within the groups