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J Biol Chem 300(4):106794

Alternatively, for a whole cell lysate, the bacterial pellet was resuspended in the chromatography buffer, lysed by sonication with a Digital Sonifier (Branson Ultrasonics, Danbury, CT) and cleared by centrifugation

This was further modified with the Arg-Gly-Asp (RGD) peptide-containing cap and photocleavable linker via cyclodextrin-adamantine hostguest complexation, which temporally enhanced or depleted [Ca 2+ ]i (measured by Fura-2 AM probe) under NIR light by on-demand releasing Ca 2+ regulators, remotely modulating RAW 264.7 cells with the M1 or M2 response to exert immune functions, as detected by IF, qRT-PCR

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