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First-strand cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA) and semiquantitative RT-PCR was performed as described previously [44]

Conclusions In summary, our study constructed an AD-related pathway-gene network and identified (-)-Vestitol and Salviolone as novel candidate natural products for AD treatment

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Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli