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L-methylfolate is the natural substrate for single-carbon methyl transfers in the synthesis of amino acids and nucleic acids (DNA, RNA)

Western Blot Analysis Western blot analysis of the crude extracts was performed by obtaining the total protein from cyanobacteria ( A 730 = 0.3) with suspension in five volumes of cold extraction buffer containing 150 mM NaCl, 50 mM Tris-HCl (pH 7.3), 1 mM EDTA, 2% -mercaptoethanol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride (PMSF), and an equal of volume of phenol saturated with Tris-HCl (pH 7.3)

The initial product was obtained by heating the reaction mixture at 90 0C for15 minutes, which gave an absorption band with max at 460 nm

[DOI] [PubMed] [Google Scholar] 49.Kumar T., Taneja S., Sachdev H.P.S., Refsum H., Yajnik C.S., Bhandari N., Strand T.A., Study Group Supplementation of vitamin B12 or folic acid on hemoglobin concentration in children 6-36 months of age: A randomized placebo controlled trial