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Because basal homocysteine levels may affect the increase in postmethionine-load homocysteine in patients with abnormal basal homocysteinemia, the net increase in postmethionine-load homocysteine above fasting values should be taken into account in the global assessment of CBS defects

Purified samples were dried with a centrifugal vacuum concentrator and stored at 80C before analysis

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The risk factors for the development of cardiovascular diseases include: hypertension, disorders in the lipid metabolism (such as hypercholesterolemia, hypertriglyceridemia and low concentration of HDL (high-density lipoprotein)), diabetes, obesity, low physical activity, alcohol consumption, smoking, menopause and exposure to xenobiotics [70,71,72]