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Furthermore, phosphomimetic substitution (S/T E) of the 12 DNA-PK consensus sites inhibits FUS-PrLD-induced phase separation and forms fibrillated aggregates in vitro 82

Mutations conferring GSH-independence map to the protomer interface between proteins VP1 and VP3 and are consistent with GSH binding being essential for the formation of pentameric subunits critical to assembly 24,27