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To eliminate the confounding effects of exogenous serine and glycine present in standard fetal bovine serum (FBS), all amino acid starvation experiments were conducted using custom-formulated media supplemented with dialyzed FBS (Cat

Peleg et al., 2016

Homocysteine then follows one of two major pathways: it is either remethylated back into methionine using folate-derived 5-methyltetrahydrofolate (5-MTHF) or enters the transsulfuration pathway to produce cysteine and glutathione, essential for antioxidant defense