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To assess the half maximal inhibitory concentration (IC50) of FOXO4-DRI on the serum limitation-induced senescent fibroblast and the non-senescent control fibroblast, the fibroblast viability in the 2% FBS induced senescent fibroblast and 10% FBS cultured non-senescent control fibroblast with increasing concentrations of FOXO4-DRI was detected, and the cell viability curves were showed in Supplementary Fig

For estrogen pathways, GHKCu appears to influence aromatase activity in some models, with potential to lower local estrogen production where excess drives pathology

500 lL from each sample was transferred to tubes containing 500 ice-cold acetonitrile with internal standard per incubation sample

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