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Sutunkova, M.P

Remarkably, AAT facilitates the chemically difficult C-H deprotonation step so much that it is only partially rate-limiting for the half-reaction, with the chemically easier ketimine hydrolysis being partially rate-limiting.[46] 2

Systematic perturbations were introduced to the glutathione-binding site by substitution of conserved charged amino acids that form crucial electrostatic contacts with the glutathione molecule