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For amino acids measurements, supernatant was collected by centrifuging flask culture samples for 3 min at 5000 rpm followed by filtration using 0.2 m syringe filters to eliminate residual cell debris

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9.2 Atopic dermatitis An atopic dermatitis CD48/80-induced itch murine model treated with apigenin (75, 150 mg/kg) saw reduced the number of scratches and inhibited the infiltration of polymorphonuclear lymphocytes [134]