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Hippocampal neurons transfected with mRFP-P-actin were treated with vehicle (PBS), 10 -12 M Nlel-AnglV, as a positive control, or various concentrations for several stabilized analogs for 5 days in culture or for 30 minutes prior to fixation on day in vitro 12 (DVI12)

Unlike most FHA domains that recognize single P-Thr residues 45,116 , the SNIP1 FHA domain likely requires two P-Thrs in P-SF3B1 for binding

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