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Notably, Smad3 , which acts primarily downstream of the TGF-activin pathway and is predicted to drive early gene expression changes in HD 23 , was significantly upregulated in the iSPN + mut subpopulation at 8 months (Extended Data Fig

Regulated necrosis may release damage-associated molecular patterns, which induce an inflammatory response that amplifies tissue injury (necroinflammation or immunogenic cell death)

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It reduces post-injury fibrosis by modulating the expression of matrix metalloproteinases (MMPs), promotes the formation of new blood vessels, and stimulates muscle fiber regeneration