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Angiotensin II type I receptor and miR-155 in endometrial cancers: synergistic antiproliferative effects of anti-miR-155 and losartan on endometrial cancer cells

The supernatant was separated and loaded onto a column packed with Ni 2+ -NTA resin and washed sequentially with lysis buffer containing 1, 10, and 50 mM imidazole