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Background: Brain MRI atlases provide detailed anatomical parcellations, but identifying regions of atrophy that correlate with clinical severity or differentiate phenotypes in PSP is challenging

First, log into your UpCloud control panel and select Deploy a Server under the Servers section

Concentrations above the normal level of 221pmol/L are indicative of an adequate B12 status, whereas those between 150 and 221pmol/L, or often below 150pmol/L, are indicative of B12 depletion or deficiency, respectively

Protein concentration was quantified with a BCA protein assay kit (Thermo Fisher, 23227) and lysates were then mixed 1:1 with 2x Laemmeli buffer and boiled for 5 min at 95 C