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Alexa Fluor conjugated secondary antibodies were used to fluorescently label the antigens of interest and following dehydration in a series of increasing concentrations of methanol and cleared using dibenzyl ether, the membrane-bound slices were placed onto a glass microscope slide and imaged using a Zeiss LSM-800 inverted confocal microscope

Administrations are strictly intravenous, performed by healthcare professionals

The mean frequency of AMPA-mediated mEPSCs recorded from vehicle treated neurons was 3.06 0.23 Hz from 33 cells

Because the experiments conducted have found that glycine is toxic to the cells, we decided to re-think the design for the glutathione degradation pathway in order to delay the time until glycine is produced