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doi: 10.1194/jlr.M038869

Reactive oxygen species induced by bile acid induce apoptosis and protect against necrosis in pancreatic acinar cells

Lyza, Anthony W

for 6 min, following which the reaction was terminated by the addition of a mixture of 10% trichloroacetic acid and 4% methanol, vortexing for 5 s, and centrifuging at 14,000 rpm for 2 min to precipitate the protein