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After a 2 week screening period all eligible participants were randomly assigned to treatment groups receiving either a subcutaneous injection of 2.4 mg of semaglutide, 2.4 mg of cagrilintide, a combination of cagrilintide and semaglutide, a semaglutide placebo, or a cagrilintide placebo [2]

Microspheres not used for biological experimentation were washed with DI water, while those used in biological applications were further washed with Dulbeccos Phosphate-Buffered Saline (DPBS, acquired from Sigma Aldrich, catalog number P3813), was used instead of normal PBS to avoid residual crosslinking from calcium and magnesium ions present in regular PBS, which is known to interfere with the alginate hydrogel matrix 49

doi: 10.1038/s41419-023-05708-0

After dissolving your old, migrated filler with Hylase, Melanie Speed mandates a strict waiting period of 3 to 14 days for the tissue to heal before introducing the new product