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For COS-7 cells, cultures were washed with PBS-CM and uptake reactions were initiated by addition of either L-H-glutamate (50 nM final concentration) or a mixture of unlabeled L-glutamate and L- 3 H-glutamate (150 M, final concentration, 99 % unlabeled and 1% labeled)

Kuhn, V

Briefly, the pancreatic duct was cannulated and distended with 4 C collagenase/protease solution using Collagenase P (Sigma-Aldrich, USA) in 1x Hanks balanced salt solution and 0.02% bovine serum albumin

In addition, most of the research on this topic has focused on Chronic Hepatitis B Virus CHBV/HDV coinfection, with limited studies on HBV-HDV superinfection