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Although production was lower compared to glucose (30% for glutarate, 15% for l-2HG), media composition was easy and required only a buffer, a nitrogen source for growth and iron for glutarate hydroxylase

X.YaoG.YangY.HanY.YangL.ZhangY

The antibodies were diluted with DMEM supplemented with 2% serum and 25 mM NH 4 Cl

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