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Read absorbance at 450 nm using a enzyme-labeled instrument (Multiskan FC, Thermo Fisher Scientific, Inc., MA, USA)

Hu C-aA: ApoL1, a BH3-only lipid-binding protein, induces autophagic cell death

Deep, diaphragmatic breathingalso called belly breathing or abdominal breathinghas been shown to significantly decrease antioxidant-depleting cortisol and lessen oxidative stress

Adhesion assay For cell-substratum adhesion assays, 96-well tissue culture plates were coated with type I collagen (0.5 mg/ml) or fibronectin (0.1 mg/ml) in PBS at RT overnight, rinsed twice with PBS and cells were allowed to adhere to the coated surface for 30 min and 60 min respectively