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All cell lines were incubated at 37 C in a humidified atmosphere containing 5% CO

Wisniewski JR, Zougman A, Nagaraj N, Mann M

This process induces the formation of intermolecular and/or intramolecular disulfide bonds in the sulfhydryl groups of active cysteine residues in redox-sensitive proteins, including cytoskeletal proteins like actin