Traditional staining approaches, such as Oil Red O and BODIPY dyes, provided the first visualization of LDs in oocytes and embryos (207), but advances in imaging and molecular profiling permit dynamic and quantitative analyses of LD biology within reproductive contexts (69)
At present, there are still many problems in the research related to gut microbiota in ITP, such as whether the type of gut microbiota in ITP patients is statistically different from that of normal people, whether the different degree of ITP disease affects the changes of gut microbiota, whether the different ages, diets, environments, and genders of the study subjects have an effect on the gut microbiota, whether the gut microbiota and its metabolites have an effect on ITP, whether the application value of gut microbiota and its metabolites in ITP is still unclear, and whether the potential clinical application value is still unclear
After 5 d of culture, cells were stained for beta-III tubulin, then counterstained with 0.1 M DAPI (D9542
Staining and quantification of the expression of cPKCs and was also determined in these samples using commercially available antibodies and did not show significant differences in PKC and expression levels in ER+ and triple negative tumors
