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After viral exposure, excess media was removed and replaced with fresh A6 media containing 10 mM BSO or 2 mM cysteine and incubated at 27C for 72 h

Oven temperature was stabilized for 5 min at 50 C

45 Likewise, sleep deprivation in both humans and animals lowers total glutathione and impairs antioxidant defenses in various tissues

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