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copri , the cultured bacterial suspension was centrifuged at 4000 rpm for 5 min, and the precipitate was washed with sterile PBS, centrifuged and resuspended in PBS (3 10 9 CFU/mL)

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Application of JA mitigated the adverse effects of alkaline stress and caused significant TSSs accumulation of 2.22 and 12.73% in 100 mM Na 2 CO 3 + 10 M JA and 150 mM Na 2 CO 3 + 10 M JA treatments, respectively, compared to only alkaline stressed plants with 100 and 150 mM 10 M JA + 0 mM Na 2 CO 3 , respectively (Table 1)