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To increase the protein recovery, the pellet from this step was resuspended in a buffer (containing 7 M urea and 2 M thiourea in 0.4 M triethylammonium bicarbonate at pH 8.5), freeze-thawed, vortexed and centrifuged at 15,000 g for 15 min at 20 C

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This is particularly relevant for professionals in Mumbai's and Bengaluru's finance and tech corridors, where long screen hours and high-stress schedules are linked with a heavier oxidative load

We then examined the network genes in diseased kidney tissue by interrogating RNA sequencing data from cortical kidney tissue from two cohorts with early and advanced DKD