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Sinomenine inhibits proliferation of SGC-7901 gastric adenocarcinoma cells via suppression of cyclooxygenase-2 expression

Methods Reagents and Antibodies Sodium arsenite (SA) (NaAsO2), Percoll, cell culture medium (DMEM/F12), antibiotics, papain, Bacterial lipopolysaccharide (LPS), sodium dodecyl sulphate (SDS), acrylamide, bis-acrylamide, TrisHCl, MTT, MK571, N-acetyl cysteine (NAC), dimethyl sulphoxide, bromophenol blue, Tween 20, -mercaptoethanol, N-ethylmaleimide, o-phthalaldehyde, EDTA, protease inhibitor cocktail, glycerol, 10 Western blocking buffer, cystine, O-phthalaldehyde (OPA), 9-fluorenylmethyl chloroformate (FMOC-Cl), 3-mercapto propionic acid (MPA), L-Cystine amino acid standard, BSTFA + TMCS, 99:1, sodium phosphate monobasic monohydrate, sodium hydroxide, boric acid, acetonitrile (LC grade), methanol (LC grade) and n-hexane were obtained from Sigma (St

The product can be searched in the Australian Register of Therapeutic Goods (ARTG) on the TGA website to confirm if its registered with TGA or no

Mechanisms and significance of eryptosis