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The biggest pros for her have been: Her old clothes fitting again Regaining confidence "Being able to enjoy looking in the mirror" has been really motivating, Wood said

Culture conditions Slices were maintained in incubators at 37 C, under a humidified atmosphere containing 5% CO2 in six-well plates containing 1 ml of slice culture medium, containing basal Earles salts medium (BME, sigma, MFCD00217343), 25% Hanks balanced salt solution (Sigma, H9394), 27 mM glucose (Sigma, G8769), 1% penicillin/streptomycin (Life technology, 15140122), 1 mM glutamine (Sigma, 228034), and 5% horse serum (New Zealand origin, heat-inactivated

69 Participants were premedicated with clonidine and midazolam, intubated using propofol and atracurium, and mechanically ventilated

Large bubbles or persistent foam suggest the reconstitution was too aggressive