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The target proteins were subsequently validated using immunohistochemistry

Neuron 25:533535 Fang Y, Schram G, Romanenko VG, Shi C, Conti L, Vandenberg CA, Davies PF, Nattel S, Levitan I (2005) Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2

This creates what researchers call pulsatile GH release short, sharp spikes in growth hormone that closely mimic your bodys own natural secretion rhythm