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After continued stirring at room temperature for four hr, the solution was filtered, and the filtrate was concentrated to dryness under vacuum at bath temperatures that were less than about 70 C

The supernatants were kept for subsequent LC-MS/MS analysis

A study out of Rome published in the Journal of the American College of Nutrition showed that infusing people with diabetes with L-carnitine improved insulin sensitivity and increased the uptake of sugar from the bloodstream

Because the compounds are structurally similar to choline (called analogues), the bacterial cells are tricked into taking them up as nutrients