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Dyson A, Dal-Pizzol F, Sabbatini G et al (2017) Ammonium tetrathiomolybdate following ischemia/reperfusion injury: chemistry, pharmacology, and impact of a new class of sulfide donor in preclinical injury models

For visualisation, the sections were stained with 3,3-diaminobenzidine (DAB) 0.03% H 2 O 2 in PBS for 10 min and then washed with demi water

That gap between expectation and physiology is precisely where protocols fail, not because the biology did nothing but because expectations were never set