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The large-scale bioreactors controlled at 34C during infection maintained a three-fold increase in productivity in the GSH elution, and excellent clearance of host cell and media impurities was observed across all batches

Results: From MarchApril 2010 200 healthy participants were enrolled

Glutaredoxin, also called thioltransferase, is encoded by the gene GLRX and protects the SH groups in the Cys residue in proteins from oxidative modification via a reduction in the reducing equivalent of GSH [14,39]

Additionally, including a purification step to separate the cleaved GST tag from the target protein, such as a second affinity chromatography step or gel filtration, can enhance the purity of the final product