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For serum samples, 75 L of chloroform:methanol extraction solvent (v:v = 1:2) was added to 20 L of mouse serum, with the exception of the serum from WT, Gclc L-KO, Nrf2 L-KO, and L-DKO mouse serum, for which 20 L of mouse serum was combined with 180 L of chloroform:methanol extraction solvent (v:v = 1:2) containing internal standards at the final concentrations: 5 nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5 nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707)

However, we question this assertion because age eligibility in a trial is not a clinical outcome, and eligibility may not correlate with improved outcomes

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The activities of DHAR and GR were also found to be increased under heat stress in temperature sensitive orchid Phalaenopsis (Ali et al., Similar to heat stress, low temperature stress also induces H 2 O 2 production in cells (Suzuki and Mittler, 2006) and is known to up-regulate transcripts, protein level and activities of different ROS-scavenging enzymes (Prasad et al., 1994