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After IVD degeneration (IDD), due to CEP calcification and reduced vertebral blood supply, the glucose concentration in the NP further decreases, with an average concentration in degenerated human NP being only 0.603 0.108 mM (Yin et al., 2020), approaching the minimum threshold required for IVD cell survival (0.5 mM) ( Under low glucose conditions, mesenchymal stem cells (MSCs) exhibit certain adaptive responses

Family and twin studies have demonstrated that OCD is a multifactorial familial condition that involves both polygenic and environmental risk factors

By integrating excitation light sources at specific wavelengths with fluorescence detection modules and employing membrane potential-sensitive fluorescent dyes, this platform holds the potential to not only continuously monitor bacterial growth curves but also to capture rapid biochemical events, such as membrane depolarization, occurring under antibiotic pressure in real time and synchronously

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