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2015, doi:10.1371/journal.pone.0122124 Gao, J., Cahill, C.M., Huang, X

Pfleger J, Gresham K, Koch WJ

In order to facilitate a reduction in steps and chemical usage for protein purification, a histidine peptide chain, or his-tag, was added to the recombinant protein expressions via genetic engineering 9,10,11,12