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The supernatants were centrifuged at 10,000 g for 15 min at 4C, and the mitochondrial pellets were dissolved in sodium dodecyl sulfate (SDS) sample buffer, subjected to 15% SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and analyzed by immunoblotting with monoclonal antibodies against cytochrome c and Cox IV

IGFBP5 plays an active role in myoblast differentiation by binding to insulin growth factor II and upregulating its expression 71

STING activation reprograms tumor vasculatures and synergizes with VEGFR2 blockade