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Cells were cultured under sterile conditions at 37 C, 5% CO and 95% humidity and used for experiments at 7080% confluence

When the size of tumor reached 100 mm 3 , the mice were intravenous injected with PBS, Ru(II)-OH, NP2, and NP4 at the dose of 2 mg Ru/kg

Eight healthy volunteers also made a one-time donation of their blood for preclinical studies to establish a baseline against the clinical trial T2DM subjects

GHK-Biotin's demonstrated capacity to promote angiogenesis and stimulate the production of VEGF means that consistent topical application may improve the density and functional capacity of the capillary network supplying each follicle