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A panel of published siRNA screens have demonstrated that a surprisingly high percentage of human TRIMs appear to regulate autophagy in cells under basal autophagy conditions (Mandell et al., 2014) or in response to autophagy induction by mTOR inhibition (Mandell et al., 2014

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Moreover, pretreated cells displayed higher basal GCLC levels than cells without pretreatment, and the second NFT treatment led to a relatively modest additional increase (Fig