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60 Thymoquinone (TQ), a major bioactive compound in Nigella sativa , exhibited significant anticancer activity against COS31 cells at a concentration of 25 M

This is because the complex of B12 and intrinsic factor is absorbed via a limited number of special receptors in the intestinal mucosa

THP-1 cells were cultivated in DMEM/F-12 GlutaMAX medium (Gibco, 10565018) supplemented with 20% (v/v) FBS and 1% (v/v) PenicillinStreptomycin under standard incubator conditions (37 C, 5% CO)

Human SSCs have been confirmed in humans and validated through xenotransplantation into sterile mice, where they successfully migrated to the niche within seminiferous tubules housing SSCs