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Description

Effects of insulin and LongR(3) on serum-free Chinese hamster ovary cell cultures expressing two recombinant proteins

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Cells were assigned to G1, S, and G2 phase based on PI fluorescence histograms analyzed with the FlowJo v10.8.1 cell cycle analysis tool and Dean-Jett-Fox model as described ( Western blotting All cells were seeded in 6-well plates to obtain 70-80% confluence at time of harvest

Statistical analysis was performed with unpaired Student's t test for the comparison between two groups and one-way ANOVA with Tukey's multiple comparisons post hoc test for the comparison between multiple variables by using GraphPad Prism 8 (GraphPad Software, Inc