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Leaf cells are filled with chloroplasts which are the organelles where photosynthesis takes place and contain specialized pigments defined as chlorophylls

Western blot Total protein levels of CHCHD2 (1:500, NBP1-94106, Novus Biologicals, Centennial, CO)), the glutamate-cystine antiporter xCT (1:500, ab37185, Abcam, Cambridge, UK), GPx-1/2 (1:100, sc-133160, Santa Cruz Biotechnology, Dallas, TX), GPx-4 (1:100, NBP2-75511, Novus Biologicals, Centennial, CO), and matrix metalloproteinase 2 (MMP-2) (1:500, 10373-2-AP, Proteintech Group, Rosemont, IL) were analyzed using western blot

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Upon reaching 70-80% confluence, they were exposed to palmitate (PA) (300 M