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Finally, -diversity distance matrixes were analyzed using PERMANOVA in QIIME2, whilst the visualization of both - and -diversity was performed via integrating the packages QIIME2R [43] and ggplot2 [44]

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Increased levels of miR-141 expression in hepatocellular carcinoma cells such as HepG2, SMMC-7721, and HuH7 cell lines has shown to downregulate Keap1 expression via the Keap1 3 untranslated region (3 UTR), resulting in transcriptional activation of Nrf2-dependent HO-1 gene (Shi et al., 2015)

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