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The precleared lysates were incubated with 2 l FLAG (Sigma, F7425) antibody for 1 h, subsequently 20 l of beads were added and immunoprecipitation was performed overnight

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of residual GSH in the test tube)/(10 min) * [(Total volume (ml)/ Volume of sample (ml)] * D.F (2) Standard curve To establish a standard curve for the modified GPx-DTNB method, we diluted the glutathione standard with 0.1 M phosphate buffer (pH 7.0) to create a 5 to 500 M concentration range, as shown in Fig

Whereas the majority of protein engineering focuses on enhancing the functions of existing proteins, often using targeted mutations, de novo protein design focuses on proteins with amino acid sequences not found in nature