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Immunoprecipitation Dynabeads Protein G were washed twice with citrate-phosphate buffer (25 mM citric acid, 50 mM Na 2 HPO 4 , pH 5.0), and incubated with FUS/TLS antibody for 1 h at room temperature

Muller F, et al

Carotenoids exhibit chemopreventive effects by suppressing the harmful effects of free radicals that regulate cancer cell proliferation, cell cycle progression, invasion, inflammation, and angiogenesis by regulating several molecular events including Akt/PI3K/mTOR, cyclin/cyclin-dependent kinase (CDK), PPAR, Wnt, VEGF, MMPs, and NF-B signaling [207]

Normally, the HPA axis is activated in response to stressors and ensures an organism responds appropriately to ever-changing environmental challenges, which is essential for survival