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So after the injection of blood concentration improve slowly, about 24 to 48 hours blood medicine concentration to peak

This steady-state ROS formation is balanced by a similar rate of consumption by the antioxidant defences provided by enzymes (e.g., superoxide dismutase, catalase, glutathione peroxidase, glutathione-5-transferase [GST]) and free-radical-scavenging endogenous and exogenous compounds (e.g., glutathione, ascorbate, -tocopherol, -carotene).5 Oxidative stress results from an imbalance between endogenous pro-oxidant formation and neutralization by antioxidant defences

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In our study, we simulated the injury process of ph-IOP during the onset of glaucoma by perfusing the mouse anterior chamber to mimic IOP elevation and returned the pressure to a normal level at the end of the designated time period