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10.2337/db23-0430 Summary Keywords mitochondrial-driven diseases, preclinical models, cybrid, conplastic mouse, mitochondrial-nuclear eXchange (MNX) mice, mitochondrial replacement therapy (MRT), CRISPR/Cas9, organoid Citation Miller DM, Archer SL and Dunham-Snary KJ (2025) Preclinical models of mitochondrial dysfunction: mtDNA and nuclear-encoded regulators in diverse pathologies

Fragment library preparation and RNA sequencing Following poly(A) selection of RNA samples purified as above, strand-specific cDNA libraries were prepared using Illuminas TruSeq stranded mRNA kit (100 bp paired-end) following the manufacturers directions, then sequenced on Illumina HiSeq 2000 with parallel samples from young and old heads multiplexed in the same lane

Biometals 27 , 495505 (2014)

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