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In separate radioactive kinase assays with [ 33 P]--ATP, we incubated recombinant GST-tagged ArfGAP1 deletion mutants (WT or F1-F5) with purified full-length FLAG-tagged LRRK2 (WT, G2019S or D1994A) derived by anti-FLAG IP from transfected SH-SY5Y cells, as described [75]

These findings suggest that targeting oxidative stress via these molecular pathways like MAPK, TLR4/NF-B, Nrf2, and PI3K/Akt may offer new therapeutic strategies for managing IBD