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doi: 10.1007/978-3-031-28202-7_1 60 WangYJWangPYanZZhouQGunturkunFLiPet al

Recognizing the musculoskeletal manifestations of vitamin D deficiency

Human induced pluripotent stem cell culture Human induced pluripotent stem cells (hiPSCs) lines, IMR90 (IMR90-4/WISCi004-B, RRID:CVCL_C437, WiCell), M001 (SCTi002-A, RRID:CVCL_9S50, STEMCELL Technologies Inc.), and Gibco (TMOi001-A, RRID:CVCL_RM92, Gibco Episomal hiPSC Line, Gibco) were routinely cultured on 5 g/mL human laminin 521 (Biolamina)-coated culture-treated six-well plates (CoStar), maintained in mTeSR Plus (STEMCELL Technologies) supplemented with 1% (v/v) Pen-Strep