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After washing, cells were incubated with 4,6-diamidino-2-phenylidole (DAPI, 10 mg/mL

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In cardiac cells, the physical proximity between mitochondria and the sarcoplasmic reticulum (SR, a specific type of ER in heart and skeletal muscle cells) allows the exchange of Ca 2+ in local microdomains, providing rapid Ca 2+ buffering