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RAW264.7 Macrophage Cell Culture and LPS-Induced Nitric Oxide Production RAW264.7 cells, a murine macrophage cell line (IBMS, CAMS/PUMC, Beijing China), were cultured in Dulbeccos modified Eagles medium high glucose (Hyclone, Logan, UT, USA) medium with 10% fetal bovine serum (HyClone, Logan, UT, USA), 100 U/ml penicillin, and 100 g/ml streptomycin in a humidified atmosphere containing 5% CO 2 at 37C

PRC2.1 MTF2 displayed an apparent K d for DNA binding of 3.4 nM, and the apparent K d was reduced by 2 to 3 folds to 9.2 nM for PRC2.1 MTF2-EPOP (Fig

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