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Similarly, the RPM supplementation for cows during late pregnancy induced a rapid maturation of gluconeogenesis and fatty acid oxidation pathways in the offsprings liver at birth, which could favor the metabolic demands in postnatal life (Jacometo et al., 2016)

The list of differentially expressed genes was uploaded to Ingenuity Pathway Analysis (QIAGEN IPA), and core analysis yielded a list of canonical pathways, of which the top 10 upregulated and downregulated pathways were visualized on bar plots using ggplot v3.5.0 [29]

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For nucleotide synthesis, we first quantified metabolites related to purine and pyrimidine metabolism by liquid chromatography-mass spectrometry (LCMS) after glutamine starvation, but no suggestive change was found in U87 or T98 GBM cells (Additional File 2: Supplemental Fig